General Information

  • ID:  hor002528
  • Uniprot ID:  A1A5Q5
  • Protein name:  N-Tyr-MIF-1
  • Gene name:  Kdm4d
  • Organism:  Rattus norvegicus (Rat)
  • Family:  JHDM3 histone demethylase family
  • Source:  animal
  • Expression:  NA
  • Disease:  NA
  • Comments:  NA
  • Taxonomy:  Rattus (genus), Murinae (subfamily), Muridae (family), Muroidea, Myomorpha (suborder), Rodentia (order), Glires, Euarchontoglires (superorder), Boreoeutheria, Eutheria, Theria, Mammalia (class), Amniota, Tetrapoda, Dipnotetrapodomorpha, Sarcopterygii (superclass), Euteleostomi, Teleostomi, Gnathostomata, Vertebrata, Craniata (subphylum), Chordata (phylum), Deuterostomia, Bilateria, Eumetazoa, Metazoa (kingdom), Opisthokonta, Eukaryota (superkingdom), cellular organisms
  • GO MF:  GO:0003684 damaged DNA binding; GO:0016491 oxidoreductase activity; GO:0031490 chromatin DNA binding; GO:0032452 histone demethylase activity; GO:0032454 histone H3K9 demethylase activity; GO:0046872 metal ion binding; GO:0051213 dioxygenase activity; GO:0140684 histone H3K9me2/H3K9me3 demethylase activity
  • GO BP:  GO:0000724 double-strand break repair via homologous recombination; GO:0001932 regulation of protein phosphorylation; GO:0006325 chromatin organization; GO:0006338 chromatin remodeling; GO:0006954 inflammatory response; GO:0006974 DNA damage response; GO:0008150 biological_process; GO:0010468 regulation of gene expression; GO:0071479 cellular response to ionizing radiation; GO:2001034 positive regulation of double-strand break repair via nonhomologous end joining
  • GO CC:  GO:0000785 chromatin; GO:0005634 nucleus; GO:0005721 pericentric heterochromatin; GO:0035861 site of double-strand break

Sequence Information

  • Sequence:  YPLG
  • Length:  4
  • Propeptide:  MKTKSTCAQNPNCSIMIFRPTKEEFNDFDKYIAYMESQGAHRAGLAKVIPPKEWRARQSYDNISNILIATPLQQVVSGQAGVFTQYHKKKKAMTVGQYRHLANSKKYQTPPHLDFEDLERKYWKNRLYESPIYGADVSGSLFDGKTQQWNVGHLGTIQDLLEQECGIVIEGVNTPYLYFGMWKTSFAWHTEDMDLYSINYLHFGQPKTWYAVPPEHGRRLELLAKELFPGSSQGCQAFLRHKVALISPTVLKENGIPFGRITQEAGEFMVTFPYGYHAGFNHGFNCAEAINFATPRWIDYGKVASQCSCGEARVSFSMDAFVRILQPERYEMWKRGQDQAVVDHTEAMGPTSQELTTWRVIQAPRKTWGLKHLRLRQVSRCLLPVATDSNIANNTQMCHTSRQAADSKGDEVQESDPAIAPPYPLGLSSPGHMSTGKRGLGRRPCELGVQESTNGAPVKRRLPEGRDDRSPSPELQSQSVTGDLIVNSDLVNPGPQHPVTASEGGLTSDP
  • Signal peptide:  NA
  • Modification:  NA
  • Glycosylation:  NA
  • Mutagenesis:  NA

Activity

  • Function:  Histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-4', H3 'Lys-27', H3 'Lys-36' nor H4 'Lys-20'. Demethylates both di- and trimethylated H3 'Lys-9' residue, while it has no activity on monomethylated residues. Demethylation of Lys residue generates formaldehyde and succinate.
  • Mechanism:  NA
  • Cross BBB:  YES
  • Target:  NA
  • Target Unid:  NA
  • IC50: NA
  • EC50: NA
  • ED50: NA
  • kd: NA
  • Half life: NA

Structure

  • Disulfide bond:  NA
  • Structure ID:  AF-A1A5Q5-F1(AlphaFold_DB_ID)

  • Structure: (PDB_ID-from https://www.rcsb.org/; AlphaFold_DB_ID-from https://alphafold.ebi.ac.uk/; hordbxxxxxx_AF2.pdb was predicted structure by AlphaFold2; hordbxxxxxx_ESM.pdb was predicted structure by ESMFold)
    •    hor002528_AF2.pdbhor002528_ESM.pdb

Physical Information

Mass: 50221 Formula: C22H32N4O6
Absent amino acids: ACDEFHIKMNQRSTVW Common amino acids: GLPY
pI: 6.09 Basic residues: 0
Polar residues: 2 Hydrophobic residues: 1
Hydrophobicity: 12.5 Boman Index: 572
Half-Life: 2.8 hour Half-Life Yeast: 10 min
Half-Life E.Coli: 2 min Aliphatic Index 97.5
Instability Index: 3835 Extinction Coefficient cystines: 1490
Absorbance 280nm: 496.67

Literature

  • PubMed ID:  20488055
  • Title:  Evidence for Conversion of N-Tyr-MIF-1 Into MIF-1 by a Specific Brain Aminopeptidase